在在有 non-B 的一个病人的肝炎 B 病毒 DNA 的 X 区域的开始 codon 的一个变化， non-C 长期的肝炎

• 作者: Akihito Tsubota Hisao Tajiri Keiko Tatsuzawa Kiyotaka Fujise Midori Kono Minoru Niiya Norio Tada Sadayori Hoshina Yoshihisa Namiki
• 来源期刊: 世界肝病学杂志：英文版（电子版）
• 学科: 医学
• 关键词: HEPATITIS B virus X region Mutation Non-B non-C chronic HEPATITIS Occult infection
• 年，卷（期）: 2011,（2）
• 页码范围: 56-60
• 页数: 5页
• 分类号: R512.62

摘要

There are cases of hepatitis involving occult hepatitis B virus(HBV)infection in which,even though the HB surface antigen(HBsAg)is negative,HBV-DNA is detected by a polymerase chain reaction(PCR).We con-ducted a sequence analysis of the entire HBV region in a case of non-B non-C chronic hepatitis in a 46-yearold female.A diagnosis of non-B non-C chronic hepatitis was made.Although HBV markers,such as HBs antibody(anti-HBs),anti-HBc,HBeAg and anti-HBe,were negative,HBV-DNA was positive.Nested PCR was performed to amplify the precore region of HBV-DNA and all remaining regions by long nested PCR.Sequence analysis of the two obtained bands was conducted by direct sequencing.Compared with the control strains,the ATG(Methionine)start codon in the X region had mut ated to GTG(Valine).It is assumed that a mutation at the start codon in the X region may be the reason why HBV markers are negative in some cases of hepatitis that involve occult HBV infection.