Structural rearrangements of polymeric insulin-loaded nanoparticles interacting with surface-supported model lipid membranes

• 作者: Bengt Kasemo Bernardino Cerda Christian Grandfils Rickard Frost Sofia Svedhem
• 来源期刊: 生物材料与纳米技术(英文)
• 学科: 化学
• 关键词: nanoparticle human insulin drug delivery SUPPORTED lipid BILAYER QCM-D REFLECTOMETRY DLS zeta potential
• 年，卷（期）: 2011,（2）
• 页码范围: 180-192
• 页数: 13页
• 分类号: O6

摘要

The design and screening of nanoparticles for therapeutic applications (nanodrugs) belong to an emerging research area, where surface based analytical techniques are promising tools. This study reports on the interaction of electrostatically assembled nanoparticles, developed for non-invasive administration of human insulin, with cell membrane mimics. Interactions between the nanoparticles and differently charged surface-supported model membranes were studied in real-time with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, in some experiments combined with optical reflectometry. Based on the experimental observations, we conclude that structural rearrangements of the nanoparticles occur upon adsorption to negatively charged lipid membranes. The degree of nanoparticle deformation will have important implications for the induced release of the protein drug load. The presented results provide an example of how a surface-based experimental platform can be used for evaluation of nanosized drug carriers.