在试验性的角膜的 neovascularization 的发展的氮的氧化物 synthase 然而并非可诱导的氮的氧化物 synthase 的潜在的参与

• 作者: and Pei-Rong Lu Gao-Qin Liu Yuan Chen
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: CORNEAL NEOVASCULARIZATION NITRIC OXIDE SYNTHASE INDUCIBLE NITRIC OXIDE SYNTHASE
• 年，卷（期）: 2011,（4）
• 页码范围: 343-348
• 页数: 6页
• 分类号: R772.2

摘要

AIM: To investigate the effect of nitric oxide and its synthetase on experimental corneal neovascularization (CRNV). METHODS: CRNV was induced by alkali injury in mice, nitric oxide synthetase (NOS) was inhibited by NG-nitro-L-arginine (L-NAME) and inducible nitric oxide synthetase (iNOS) was inhibited by aminoguanidine hemisulfate salt (AG). The inhibitory effect was detected at day 2 and 4 after corneal alkali injury by reverse transcription polymerase chain reaction (RT-PCR). CRNV was compared between the control and the treated mice by microscopic observation and corneal whole mount CD31 immunostaining. RESULTS: The inhibition of L-NAME to NOS and AG to iNOS after corneal injury was confirmed by RT-PCR (P 【0.05). Compared with control mice, L-NAME treated mice exhibited significantly decreased CRNV areas (P 【0.05). In contrast, AG treatment failed to attenuate alkali induced CRNV (P 】0.05). CONCLUSION: Our findings suggest that NOS but not iNOS plays a critical role in alkali injury induced CRNV.