mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C.elegans

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2011,（11）
• 页码范围: 1605-1618
• 页数: 14页
• 语种: 英文
• DOI: 10.1038/cr.2011.102

摘要

MicroRNA (miRNA) regulates gene expression in many cellular events,yet functions of only a few miRNAs are known in C.elegans.We analyzed the function of mir-35-41 unique to the worm,and show here that mir-35 regulates the G1/S transition of intestinal cells and germ cell proliferation.Loss of mir-35 leads to a decrease of nuclei numbers in intestine and distal mitotic gonad,while re-introduction of mir-35 rescues the mutant phenotypes.Genetic analysis indicates that mir-35 may act through Rb/E2F and SCF pathways.Further bioinformatic and functional analyses demonstrate that mir-35 targets evolutionaily conserved lin-23 and gld-1.Together,our study reveals a novel function of mir-35 family in cell division regulation.