由抵销 anti-SDF-1 抗体的禁止的试验性的角膜的 neovascularization

• 作者: Gao-Qin Liu, Xue-Guang Zhang
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: CORNEAL NEOVASCULARIZATION ALKALI injury CHEMOKINE
• 年，卷（期）: gjykzzywb_2012,（1）
• 页码范围: 7-12
• 页数: 6页
• 分类号: R779.1

摘要

AIM: To explore the effect of SDF-1α on the development of experimental corneal neovascularization (CRNV).METHODS: CRNV was induced by alkali injury in mice. The expression of SDF-1α and CXCR4 in burned corneas was examined by Flow Cytometry. Neutralizing anti-mouse SDF-1α antibody was locally administrated after alkali injury and the formation of CRNV 2 weeks after injury was assessed by Immunohistochemistry. The expression of VEGF and C-Kit in burned corneas was detected by RT-PCR.RESULTS: The number of CRNV peaks at 2 weeks after alkali injury. Compared to control group, SDF-1α neutralizing antibody treatment significantly decreased the number of CRNV. RT-PCR confirmed that SDF-1α neutralizing antibody treatment resulted in decreased intracorneal VEGF and C-Kit expression.CONCLUSION: SDF-1α neutralizing antibody treated mice exhibited impaired experimental CRNV through down regulated VEGF and C-Kit expression.