Study on Biological Characteristics and Toxicity Determination of Colletotrichum orbiculare Causing Anthracnose of Watermelon in Hainan Province

• 作者: ZHOU Wen-jing
• 来源期刊: 植物病虫害研究：英文版
• 学科: 农学
• 关键词: 植物病虫害 预防 植物保护 植物检疫
• 年，卷（期）: 2012,（1）
• 页码范围: 5-9
• 页数: 5页
• 分类号: S4

摘要

[Objective] This paper was aimed to study the biological characteristics of Colletotrichum orbiculare(Berk.et Mont.) Arx, and accomplish the preliminary screening the test of fungicides. [Method]The pathogen was isolated from the infected leaves of watermelon collected from greenhouse in Hainan Province. The biological characteristics of C. orbiculare and indoor toxicity of fungicides were studied. [Results] The mycelium grew fastest on the medium with leaf extract of watermelon. The optimum temperature for mycelial growth and spore germination was 28 and 30 ℃, and the optimum pH was 7-9 and 6-8, respectively. The continuous darkness condition was most suitable for mycelial growth. The lethal temperature of spore was 55 ℃ water bath for 10 min in water bath. The suitable carbon sources were maltose, L-arabinose and glucose, and the suitable nitrogen sources were yeast extract, beef extract and peptone. In the indoor toxicity determination, 32.5% Benzoic azoxystrobin SC, 50% Dichlorotetrakis WP and 10% Difenoconazole WG showed better inhibition effects against the mycelial growth of C. orbiculare, with the EC50 values of 0.018 0, 0.060 2 and 0.471 5 μg/ml, respectively. [Conclusion]The research provided theoretical basis for the control of watermelon anthracnose in watermelon fields.