Calcium Integrin Binding Protein Associates with Integrins <i>α<sub>V</sub>β<sub>3</sub></i>and <i>α<sub>IIb</sub>β<sub>3</sub></i>Independent of <i>β<sub>3</sub></i>Activation Motifs

• 作者: Innocent H. Yamodo Scott D. Blystone
• 来源期刊: 细胞生物学(英文)
• 学科: 医学
• 关键词: Leukocyte INTEGRIN Cytoskeleton Hematopoietic ACTIVATION Signaling
• 年，卷（期）: 2012,（2）
• 页码范围: 30-37
• 页数: 8页
• 分类号: R73

摘要

The Calcium Integrin Binding protein (CIB) has been identified as interacting specifically with the cytoplasmic tail of the integrin αIIb domain to induce receptor activation and integrin αIIbβ3 mediated cell adhesion to extracellular proteins. In K562 cells stably expressing mutated integrin αVβ3 or chimeric αVβ3 carrying αIIb cytoplasmic tail, we report that the interaction of CIB with β3 integrins is not αIIbβ3 specific but binds αIIb as well as αV cytoplasmic tail domains. A double mutation of two proline residues to alanine residues in the αIIb cytoplasmic domain, previously shown to disturb its conformation, inhibits chimeric αV/αIIbβ3-CIB interaction. This demonstrates that αIIb cytoplasmic domain loop-like conformation is required for interaction with CIB. Moreover, mutations of β3 cytoplasmic domain residues Tyr-747 and/or Tyr-759 to phenylalanine residues (Y747F, Y759F, and Y747,759F) as well as residues Ser-752 to proline or alanine (S752P and S752A), do not affect the αIIbβ3 or αVβ3 interaction with CIB. Since tyrosine residues Tyr-747 and/or Tyr-759 are the sites of tyrosine phosphorylation of β3 subunit, these results suggest that the β3 integrin-CIB interaction occurs through aβ3-phosphorylation independent mechanism. Likewise, ablation of conformation-dependent affinity change in β3 Ser752Pro mutation had no affect on CIB-β3 interaction. In summary, our results demonstrate that the αIIb-subunit integrin and CIB interaction is non-exclusive and requires the loop-like αIIb-cytoplasmic domain conformation. An interaction of CIB with αV-containing integrins provides an additional role for this molecule in keeping with its expression outside of platelets.