The CD133/1<sup>+</sup>cell subset from human subcutaneous adult fat retains hemogenic potential

• 作者: Camila Santos de Moraes Claudia Sondermann Freitas Daniel Fabiano Ferreira Eliana Abdelhay Fernando Serra Paulo Roberto Albuquerque Leal
• 来源期刊: 干细胞探索(英文)
• 学科: 医学
• 关键词: ADIPOSE Tissue-Derived Stem Cells CD133 Hematogenesis Hemangioblast PERICYTE
• 年，卷（期）: 2012,（2）
• 页码范围: 36-40
• 页数: 5页
• 分类号: R73

摘要

Research has shown that cells from adult fat tissue can effect long-term blood reconstitution. Fat-derived multipotentiality was ascribed to CD34+ perivascular populations from its prominent microvasculature, that represent mostly non-hemogenic, mesenchymal cells, although this tissue contains a CD34+45+ subset committed to a hemogenic fate. Here, in order to analyze cell subsets presenting hemogenic capabilities within fat, CD133/1+ and pericytes, the latter defined by CD140b (PDGFRb, Platelet-Derived Growth Factor Receptor Beta) expression, were immunomagnetically selected from stromal-vascular fractions (SVF). In Vitro Colony Forming Unit (CFU) assays were negative for CD140b+ pericytes and positive for CD133/1+ cells when a prolonged CFU assay was performed, revealing fat as another store of primitive progenitors that retain hemogenic potential.