ImmunogenicCharacteristicsofRSVF2ProteinExpressedinE.coliRosetta(DE3)

• 作者: HUANGFen JINGShenRong WANGYuNing YUETingTing ZENGWeiKun
• 来源期刊: 昆明理工大学学报（自然科学版）
• 学科: 医学
• 关键词: respiratorysyncytialvirus immunogeniccharacteristic F2protein
• 年，卷（期）: 2012,（2）
• 页码范围: 54-60
• 分类号: R373.1
• 语种: 中文

摘要

Respiratorysyncytialvirus(RSV)isamajorcauseoflowerrespiratorytractinfectioninchildrenyoungerthan5yearsold.However,asafeandeffectivevaccinehasnotbeendevelopedsofar,thereforeitisnecessarytoemploydifferentstrategiestoacceleratethedevelopmentofpreventativevaccines.Inthisstudy,thegenederivedfromRSVfusion(F)proteinF2subunitisexpressedinE.coliRosetta(DE3)usingrecombinantglutathioneStransferase(GST)taggedprotein(GSTF2)ofvector.ExpressionofrecombinantproteinisidentifiedbySDSPAGEandWesternBlot.KunmingmiceareimmunizedwithpurifiedF2protein.SeraarecollectedafterthreetimesboosttoassesstheimmuneresponsebyELISA.MiceimmunizedwithrecombinantproteinyieldhightitersofIgG(p<0.05),andtheratioofIgG1/IgG2aisalsosignificantlyhigherthanthatofcontrol.However,thereisnosignificantchangeinsecretaryIgA(sIgA)intherespiratorytractmucosallavagefluidofmice(p>0.01).ResultsshowthattheF2proteinisapotentialvaccinecandidateforRSV,whichinducesTh2typeimmuneresponse.