Organic Solvent-Free and Simple Method for Determining Cyromazine and Its Metabolite, Melamine, in Cow’s Milk

• 作者: Naoto Furusawa
• 来源期刊: 分析科学方法和仪器期刊(英文)
• 学科: 化学
• 关键词: CYROMAZINE MELAMINE HPLC-PDA Milk: ORGANIC SOLVENT-FREE
• 年，卷（期）: fxkxffhyqqkyw,（2）
• 页码范围: 68-73
• 页数: 6页
• 分类号: O6

摘要

This paper described an organic solvent-free, rapid, simple, and space-saving method of sample preparation followed by HPLC coupled photo-diode array (PDA) detector for simultaneous quantification of cyromazine (CYR) and its decy-cropropylated metabolite, melamine (MEL), in milk. The HPLC-PDA was performed on an Inertsil? HILIC column with an isocratic aqueous mobile phase. Analytes were extracted from the sample using water, and purified by Mono-Spin?-C18, a centrifugal monolithic silica spin mini-columns, and quantified within 20 min. The method, performed under 100% aqueous conditions, obtained average recoveries for CYR and MEL in the range of 93.2% - 99.1% with relative standard deviations ≤ 2.8%. The quantitation limits were 8.5 ng/mL for CYR and 10 ng/mL for MEL, respectively. No organic solvents were used at any stage of the analysis.