人的脐的间充质的干细胞和它的 adipogenic 区别: 由原子磁性的回声光谱学介绍

• 作者: Zhi-Feng Xu, Ai-Zhen Pan, Fang Yong, Chong-Yang Shen, Yao-Wen Chen Ren-Hua Wu
• 来源期刊: 世界干细胞杂志：英文版（电子版）
• 学科: 医学
• 关键词: Human UMBILICAL MESENCHYMAL stem cell Adipogenic DIFFERENTIATION MR SPECTROSCOPY BIOMARKER
• 年，卷（期）: 2012,（4）
• 页码范围: 21-27
• 页数: 7页
• 分类号: R329

摘要

AIM:To study the metabolic profile of human umbilical mesenchymal stem cells(HUMSC) and adipogenic differentiation by nuclear magnetic resonance(NMR) spectroscopy.METHODS:HUMSC isolated from human umbilical cord stroma were induced to adipocytes over 2 wk by adding dexamethasone,3-isobutyl-1-methylxanthine,indomethacin,and insulin to the culture medium.Adipogenic differentiation was confirmed by Red O staining and transcription-polymerase chain reaction.Perchloric acid extracts of the HUMSCs and adipocytes(about 7 ×10 6) were characterized for metabolites by using in vitro high resolution 9.4T NMR spectroscopy.RESULTS:Several major metabolites,such as:choline,creatine,glutamate and myo-inositol,acetate,and some fatty acids/triglycerides,were observed in the MR spectroscopic pattern of HUMSCs and their adipogenic differentiation.HUMSCs are characterized by an unusually low number of NMR-detectable metabolites,high choline,acetate,glutamate and creatine content.However,the metabolic profiles of adipogenic differentiation demonstrated considerably higher methionine and fatty acids,and non-detectable creatine.CONCLUSION:The biomarkers of HUMSCS and adipocytes were obtained and assigned.NMR spectroscopy will be a promising tool for monitoring stem cell differentiation.