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摘要:
The transfer of proteins and nucleic acids from donor to acceptor cells via small membrane vesicles has been implicated with (patho)physiological consequences. Previously the upregulation of esterification and downregulation of lipolysis in small rat adipocytes upon incubation with exosomes and microvesicles (EMVs) released from large adipocytes and harbouring the glycosylphosphatidylinositol (GPI)-anchored proteins, Gce1 and CD73, transcripts specific for FSP27 and GPAT3, and microRNAs, miR-16 and miR-222 was demonstrated. Here the release of EMVs from large (but not small) primary and differentiated and human rat adipocytes in response to palmitate, H2O2 and the anti-diabetic sulfonylurea, glimepiride, is shown to be significantly reduced upon inhibition of histone H3 lysine9 methyltransferase G9a by trans-2-phenylcyclopropylamine (tPCPA) and histone H3 lysine4 demethylase LSD1 by BIX01294. Inhibition of EMV release by tPCPA and BIX01294 was not caused by apoptosis but accompanied by upregulation of the H2O2-induced stimulation of lipid synthesis and downregulation of lipolysis in large (but not small) primary and differentiated rat and human adipocytes. In contrast, the simultaneous presence of tPCPA and BIX-01294 had almost no effect on the induced release of EMVs and lipid metabolism. These findings argue for regulation of the release of EMVs harbouring specific GPI-anchored proteins, transcripts and microRNAs from rat and human adipocytes by histone H3 methylation at lysines 4 and 9 in interdependent fashion. Thus the EMV-mediated transfer of lipogenic and anti-lipolytic information between large and small adipocytes in response to certain physiological and pharmacological stimuli seems to be controlled by epigenetic mechanisms.
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篇名 Release of exosomes and microvesicles harbouring specific RNAs and glycosylphosphatidylinositol-anchored proteins from rat and human adipocytes is controlled by histone methylation
来源期刊 美国分子生物学期刊(英文) 学科 医学
关键词 cAMP EPIGENETIC Control EXOSOMES GLIMEPIRIDE GLYCOSYLPHOSPHATIDYLINOSITOL Lipid Metabolism MICROPARTICLES miRNA MICROVESICLES
年,卷(期) 2012,(3) 所属期刊栏目
研究方向 页码范围 187-209
页数 23页 分类号 R73
字数 语种
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cAMP
EPIGENETIC
Control
EXOSOMES
GLIMEPIRIDE
GLYCOSYLPHOSPHATIDYLINOSITOL
Lipid
Metabolism
MICROPARTICLES
miRNA
MICROVESICLES
研究起点
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研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
美国分子生物学期刊(英文)
季刊
2161-6620
武汉市江夏区汤逊湖北路38号光谷总部空间
出版文献量(篇)
191
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0
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0
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