[Objective] To isolate and preserve a high-activity Douchi fibrinolytic enzyme producing strain from Douchi products.[Method] The Douchi fibrinolytic enzyme producing strain was screened on the selected medium prepared with self-made pork blood powder,the strain with the highest activity was screened out according to the size of hydrolysis cycle,and then preserved in LB medium.[Result] A Douchi fibrinolytic enzyme producing strain with high thrombolytic activity was successfully screened out from the Douchi produced in Hunan,Guangdong and Jiangxi Provinces.[Conclusion] The study lays foundation for the development of new-type thrombolytic drugs.