Community Diversity of Associated Bacteria of Radopholus similis

• 作者: Fen ZHENG Meiying FU Miancai CHEN Zhixiang ZHAO
• 来源期刊: 植物病虫害研究：英文版
• 学科: 生物学
• 关键词: 香蕉穿孔线虫 细菌群落 群落多样性 DNA克隆 ARDRA 系统发育树 序列相似性 DNA文库
• 年，卷（期）: 2012,（2）
• 页码范围: 33-37
• 页数: 5页
• 分类号: Q78

摘要

[Objective] The paper was to reveal the species abundance of associated bacterial community of Radopholus similis, and the diversity of its bacteria. [Methods] Through the construction of 16S rDNA cloning library of bacteria, the positive clone was carried out ARDRA analysis, and the phylogenetic tree was constructed. [Results] OUT (Operational Taxonomic Units) was classified according to sequences similarity of 97%, and the constructed 16S rDNA library of bacteria contained 13 OTUs, belonging to Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria, respectively. The dominant species was Betaproteobacteria, especially Burkholderia was the dominant bacterium. [Conclusions] The diversity of associated bacteria of R. similis was high, and these bacteria may have certain ecological significance to R. similis.