Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal

• 作者: Nobuo Goto Shin-ichiro Yanagiya
• 来源期刊: 表面工程材料与先进技术期刊(英文)
• 学科: 医学
• 关键词: LASER CONFOCAL MICROSCOPY ATOMIC FORCE MICROSCOPY Surface Process Protein CRYSTAL
• 年，卷（期）: 2012,（3）
• 页码范围: 210-214
• 页数: 5页
• 分类号: R73

摘要

We demonstrated the insitu observation of a moving atomic force microscope (AFM) cantilever using a laser confocal microscope combined with a differential interference microscope (LCM-DIM). The AFM cantilever scanned or indented the {110} surface of a hen egg-white lysozyme crystal in a supersaturated solution. Using a soft cantilever, we could observe the step growth with high time resolution by LCM-DIM and perform quantitative measurements of the step height by AFM simultaneously. In addition, a hard cantilever was used with LCM-DIM to observe the dynamics of crystal surface scratching and indentation. In the supersaturated solution, the small steps generated from the scratched line aggregated to macro steps, and subsequently flattened the surface.