[Objective] The paper was to understand whether abscisic acid (ABA) participates the defense effect of hosts against Turnip crinkle virus (TCV). [Method] By external application of ABA and using the corresponding mutants, TCV coat protein levels of various plants were detected by western-blot, and the expression of defense-related genes was analyzed by RT-PCR. [Result] After external application of SA, JA, ET and ABA, only SA and ABA could significantly inhibit TCV accumulation. In arabidopsis-TCV interaction system, SA and ABA had antagonistic effect with each other, the defense effect against TCV induced by ABA was independent on SA pathway. [Conclusion] The plants could activate distinct signal pathways to adjust the defense of hosts against virus.