GeneticandProteinSequenceAnalysisofrpS29from Ailuropodamelanoleuca (giantpanda)

• 作者: BoSong JieDeng JieZhong LiLuo Ting Wang WanruHou XiangDing YilingHou
• 来源期刊: 重庆与世界(学术版)
• 学科: 生物学
• 关键词: giantpanda rpS29 RT-PCR cloned sequenceanalysis
• 年，卷（期）: 2012,（8）
• 页码范围: 6-9
• 分类号: Q785
• 语种: 中文

摘要

ToexplorethestructuralcharacteristicofribosomalproteinS29(rpS29)geneofAiluropodamelanoleuca (giantpanda),primersweredesignedbasedontheknownnucleotidesequenceofrpS29genestoclonethecDNAand genomicsequencesofthisgenefromgiantpandabyRT-PCRandPCRstrategyrespectively,andthentheclonedcDNA andgenomicsequencesweresequencedandanalyzedpreliminarily.TheresultsindicatedthatthecDNAfragmentofthe rpS29fromthegiantpandais205bpinsizeinlength,containinganopenreadingframe(ORF)of171bp,encoding56 aminoacids.Thelengthofthegenomicsequenceis1598bp,withthreeexonsandtwointrons.Thecodingsequence showsahighdegreeofhomologytothoseof Homosapiens,Bostaurus,Rattusnorvegicusand Musmusculus with 75%,94.74%,89.47%and88.89%respectively,Whileallthehomologiesforaminoacidsequencesarehighupto 100%.PrimarystructureanalysisrevealedthatthemolecularweightoftheputativerpS29proteinis6.68KD,witha theoreticalpIof10.63.Basedontopologyprediction,therearethreedistincttypesoffunctionalsitesintherpS29 proteinofgiantpanda.