By taking 65 counties and cities of Jiangsu Province as empirical units, rurality indexes have been constructed based on regional urban-rural integration, and a quantitative evaluation has been conducted on the rurality of Jiangsu Province based on county scale. With the support of ArcGIS 9.3, GeoDA 095 and SPSS 16, by adopting Jenks best breakpoint classification and spatial autocorrelation analysis, quantitative analysis and research have been conducted on the spatial differentiation of rurality of Jiangsu Province. The results show that the rural difference of Jiangsu Province based on county scale is not remarkable, and the area with strong rurality takes a larger proportion; rurality increases from South Jiangsu to North Jiangsu progressively; rurality concentrates in general, seven counties and cities including Jiangyin and Kunshan City are "cold points" while seven counties and cities including Guanyun and Funing City are "hot points" of rurality.