A field study was conducted to determine the effect of Trinexapac-Ethyl (TE) on four wheat varieties (Rafi C97, Altar C84, Nacori C97 and Rayon F86) at three growth stages (first node, second node and flag leaf). TE application (150 g a.i. ha-1) reduced plant height and decreased grain yield at any growth stage. Yield decrease was associated to a significant reduction in the number of grains per spike and in grain weight. TE delayed flowering time and grain maturity, while stem diameter and spikes m-2 were not affected. Further studies are needed to clarify the effect of TE on wheat under desert conditions of Mexico.