[Objective] The aim was to study the biodiversity and its protection measures in Qinling. [Method] The current situation and protection measures of the biodiversity in Qinling area were reviewed by the perspective of eco-safety and the eco-value. [Result] Qinling is the natural gene bank for the study of the origin and development of living beings. Forest ecological system is the main body, with many ecological systems and complete vertical spectrometer. The plants have various varieties, complicated components, rare species, rich special plant and old heritance. There were 722 species of vertebrates, including 162 species in 16 families of 7 orders of fish, 26 species in 8 families of 2 orders of amphibian, 44 species in 8 families of 2 orders of reptiles, 373 species in 53 families of 17 orders of birds, and 117 species in 27 families of 7 orders of animals. The number of order, family and species of vertebrates was 70%, 53.04% and 22.06%. It was suggested to formulate policy promoting biodiversity protection and sustainable development, building natural protection net system to protect biodiversity in Qinling. [Conclusion] The study provided theoretical basis for the protection of biodiversity in Qinling area.