Label-free quantitative detection using porous silicon as optical biosensor

• 来源期刊: 光电子快报（英文版）
• 年，卷（期）: 2012,（4）
• 页码范围: 314-317
• 页数: 4页
• 语种: 英文
• DOI: 10.1007/s11801-012-2244-3

摘要

The single-layer porous silicon is prepared by electrochemistry etching method,which is used as an immunosensor for determining recombinant mouse zona pellucida 3 fusion protein (r-mZP3) by Raman spectroscopy analysis at room temperature.The molecule binding increases the effective optical thickness (EOT),and thus the Raman spectrum intensity decreases.The concentration and variation of Raman intensity show a good linear quantitative relation.The excellent sensing performance could open the way to a new family of optical sensors for biological standardization.