Time sequence signals of instantaneous longitudinal and normal velocity components at different longitudinal and normal positions in a turbulent boundary layer have been finely measured simultaneously by IFA300 constant temperature anemometer and double-sensor hot-wire probe with sampling resolution higher than the frequency that corresponds to the smallest time scale of Kolmogorov dissipation scale before/after introducing artificial periodic blow/suction perturbation. The period-phase-average technique is applied to extract the periodic waveforms of artificial perturbation from instantaneous time sequence signals of longitudinal and normal turbulence background. Experimental investigation is carried out on the attenuation characteristics of periodic perturbation wave with different frequency along longitudinal direction and normal direction in a turbulent boundary layer. The amplitude distributions of longitudinal and normal disturbing velocity component for different perturbation frequencies are measured at different downstream and normal positions in turbulent boundary layer. The amplitude growth rate of artificial periodic perturbation wave is calculated according to flow instability theory. The experimental results are compared and in consistent with the theoretical and numerical results.