Amyloid precursor protein-mediated modulation of capacitive calcium entry

• 来源期刊: 科学通报(英文版)
• 学科: 医学
• 年，卷（期）: 2012,（35）
• 页码范围: 4552-4559
• 页数: 8页
• 分类号: R749.16
• 语种: 中文

摘要

Alzheimer's disease(AD) is a progressive neurodegenerative disorder.In the present study,the function of amyloid precursor protein(APP) in modulating capacitive calcium entry(CCE),a refilling mechanism for depleted intracellular calcium stores,was investigated.CCE in neural 2a(N2a) cells stably expressing wild-type human APP was lower than in wild-type N2a cells,while CCE in APP knockout mouse embryonic fibroblast(MEF) cells was higher than in their wild-type counterparts.We demonstrate that wild-type APP depresses CCE.Furthermore,using N2a cells transfected with C-terminal APP fragments,we show that these fragments anchored in the cell membrane play an important role in CCE depression.