Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method

• 来源期刊: 科学通报(英文版)
• 学科: 生物学
• 年，卷（期）: 2012,（22）
• 页码范围: 2898-2900
• 页数: 3页
• 分类号: Q511
• 语种: 中文

摘要

The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method.We achieved the two-dimensional scanning images of OIRD Re{△ p △s } intensities before and after interactions as well as the curves of reaction dynamic processes.The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays,but also can real-time monitor the kinetic processes of biomolecular interactions.