Thermostable alkaline protease production from Bacillus pumilus D-6 by using agro-residues as substrates

• 作者: Bijender Kumar Bajaj Gaytri Jamwal
• 来源期刊: 酶研究进展(英文)
• 学科: 医学
• 关键词: ALKALINE PROTEASE Agro-Residues BACILLUS pumilus THERMOSTABLE
• 年，卷（期）: myjjzyw,（2）
• 页码范围: 30-36
• 页数: 7页
• 分类号: R73

摘要

Proteases due to their wide range of applications in biotechnological processes have been the??focus of intense research for many decades. However, from industrial?application view point most of the available proteases lack desired properties;?therefore, search for better and efficient thermostable alkaline proteases are?always on.?Bacillus pumilus?D-6, isolated from dairy plant soil sample, in the?current study produced protease which showed activity and stability at high?alkaline?pH (8 - 12) and high?temperatures (70。C- 100。C). Enzyme activity remained unfazed even in presence?of inhibitors like Pb2+and Hg2+which are considered?universal inhibitors of enzyme activity. Besides, the organism successfully?utilized crude agriculture based substrates as carbon and nitrogen source and?produced substantial enzyme titre.