骨头的 Immunophenotype 和区别能力从 CBA/Ca， ICR 和 Balb/c 的导出髓的间充质的干细胞老鼠

• 作者: Yin Yin Ooi Zul'atfi Rahmat Shinsmon Jose Rajesh Ramasamy Sharmili Vidyadaran
• 来源期刊: 世界干细胞杂志：英文版（电子版）
• 学科: 医学
• 关键词: Mesenchymal stem cells Mouse bone MARROW CBA/Ca STRAIN ICR STRAIN BALB/C STRAIN IMMUNOPHENOTYPING Differentiation
• 年，卷（期）: 2013,（1）
• 页码范围: 34-42
• 页数: 9页
• 分类号: R329

摘要

AIM:To assess the capacity to isolate and expand mesenchymal stem cells(MSC)from bone marrow of CBA/Ca,ICR and Balb/c mice. METHODS:Bone marrow of tibia and femur were flushed,cultured and maintained in supplemented Dulbecco’s modified Eagle’s medium.MSC immunophenotype of cultures were tracked along increasing passages for positivity to CD106,Sca-1 and CD44 and negativity to CD45,CD11b and MHC classⅡ.Differentiation capacity of MSC towards osteogenic and adipo-genic lineages were also assessed. RESULTS:MSC were successfully cultured from bone marrow of all 3 strains,albeit differences in the temporal expression of certain surface antigens.Their differentiation into osteocytes and adipocytes were also observed. MSC from all 3 mouse strains demonstrated a shift from a haematopoietic phenotype(CD106-CD45+CD11b+Sca-1low)to typical MSC phenotype(CD106+CD45-CD11b-Sca-1high)with increasing passages. CONCLUSION:Information garnered assists us in the decision of selecting a mouse strain to generate MSC from for downstream experimentation.