Deletion of the TPM1 and MDM20 Genes Affect the Mechanical and Structural Properties of Yeast Cells

• 作者: Annette Doyle David R.Burton Francis Lilley Gary Johnston Mark F.Murphy Steven R.Crosby Terri G.Kinzy Winder B.Perez
• 来源期刊: 分子与细胞生物力学(英文)
• 学科: 医学
• 关键词: YEAST ACTIN Tpm1p Mdm20p MECHANICS Mechanomics
• 年，卷（期）: 2013,（4）
• 页码范围: 275-288
• 页数: 14页
• 分类号: R73

摘要

Many diseases including cancer are associated with a disorganisedcytoskeleton. The process of characterising how cytoskeletal disorganisation affectsthe mechanical properties of cells offers the potential to develop new drugsand treatment regimes that may exploit mechanical weakness in cells and tissues.This work investigated the role of actin associated proteins, namely tropomyosin 1(tpm1p) and mitochondrial distribution and morphology protein 20 (mdm20p), onthe mechanical and morphological properties of yeast cells. For the first time it wasshown that deletion of both the TPM1 and MDM20 genes resulted in a decreasein Young’s modulus when compared to the wild-type cells. The deletion strainsappeared to have aberrant cell walls when compared to the wild-type strain andalso appeared to have lost the characteristic elliptical morphology that is normallyexhibited by yeast. Deletion of the TPM1 gene resulted in a significant increasein mean conjugate cell diameter when compared to the wild-type cells, howeverdeletion of the MDM20 gene did not have any significant effect upon the meanconjugate diameter of the yeast cells.