Evaluation of three methods for detection of methicillinresistant Staphylococcus aureus

• 作者: Asli Gamze Sener Ilhan Afsar Mustafa Demirci Sevin Kirdar
• 来源期刊: 世界转化医学杂志
• 学科: 医学
• 关键词: CULTURE METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS MOLECULAR assays
• 年，卷（期）: sjzhyxzz,（2）
• 页码范围: 27-31
• 页数: 5页
• 分类号: R

摘要

AIM:To evaluate GenoType methicillin-resistant Staphylococcus aureus(MRSA)Direct assay and cultivation for the identification of MRSA by using mecA polymerase chain reaction(PCR)as the“gold standard”assay.METHODS:In total of 61 nasal specimens from patients at the intensive care unit were studied by Geno-Type MRSA Direct test,conventional culture method and automated bacterial identification system.The results of GenoType MRSA Direct assay were compared to conventional culture method the identification of MRSA and mecA gene PCR as the“gold standard”method.The sensitivity,specificity,positive predictive value and negative predictive value were calculated.RESULTS:In total,61 specimens were studied.Fiftyfour specimens(88.5%)were negative by all three methods.Six swabs(9.8%)were found positive by GenoType MRSA Direct test,conventional culture method and automated bacterial identification system.The presence of mecA in these strains was confirmed by PCR.One swab sample was negative for culture methods but MRSA and mecA gene were detected by Geno-Type MRSA Direct test and mecA PCR respectively.GenoType MRSA Direct test had a sensitivity of 100%(6/6)and a specificity of 100%(55/55),with a positive predictive value of 100%and a negative predictive value of 98%.Culture method of MRSA had a sensitivity of 83.3%(5/6)and a specificity of 98.2%(55/56).CONCLUSION:It was found that the GenoType MRSA Direct assay,which is a rapid and accurate test,is of the same sensitivity and specificity with mecA PCR.The GenoType MRSA Direct assay can be a better tool for rapid and accurate detection of MRSA in diagnostic laboratories.