Analysis of Functional Group of Alkaline Phosphatase from DFRKN-1

• 作者: Ma Aiying Mou Yi Yang Zhenhua Zhang Kaowen
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 生物学
• 关键词: 碱性磷酸酶活性 官能团 组氨酸残基 金属离子 有机试剂 必需基团 半胱氨酸 活性构象
• 年，卷（期）: 2013,（1）
• 页码范围: 39-41
• 页数: 3页
• 分类号: Q516

摘要

To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nematode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN-1 were analyzed in the paper. The results showed that Mg2+, Ba2+ and K+ under certain concentrations activated enzyme significantly and Zn2+ could inhibit enzyme activity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline phosphatase; sulfhydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phosphatase.