The comparison of the efficacy of swine FMD vaccine emulsified with oil adjuvant of ISA 201 VG or ISA 206 VG

• 作者: Baoxia Xie Chunxue Zhou Daliang She Dong Li Pinghua Li Pu Sun Xingwen Bai Yingli Chen Zaixin Liu
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: SWINE FMD VACCINE ADJUVANT ISA 201 ISA 206 Immune EFFICACY
• 年，卷（期）: 2013,（3）
• 页码范围: 22-25
• 页数: 4页
• 分类号: R73

摘要

The Seppic Company developed a new adjuvant Montanide ISA 201 VG, the upgraded version of Montanide ISA 206 VG, which keep the advantage and added some chemical components on the basis of ISA 206 to improve the cellular responses. The aim of the study is to compare the efficacy of swine FMD (foot-and-mouth) vaccine emulsified with oil adjuvant of ISA 201 or ISA 206 respectively. The pigs were vaccinated with FMD vaccine emulsified with inactive FMD type O antigen and adjuvant ISA 201 or ISA 206 respectively, according to 2.0 ml (1/1 dose), 0.67 ml (1/3 dose), 0.22 ml (1/9 dose) to calculate their PD50. The sera were collected from the vaccination of the day 0, 3, 7, 14, 21, 28 and the ELISA FMD type O antibody were detected. Furthermore, the PD50 were calculated after the pigs were challenged with virulent FMDV type O on 28 days post vaccination. The ELISA antibody titers of 201vaccine were significantly higher than that of 206 (except the third time). The fifty percent of protection dose (PD50) of 201 vaccine (PD50 = 15.59) was higher than that of 206 vaccine (PD50 = 10.05). The above data showed that the efficacy of the FMD vaccine emulsified with ISA 201 was better than which with ISA 206.