MMP-8 analysis in gingival crevicular fluid using ELISA and novel chair-side test

• 作者: Bangalore Varadhan Karthikeyan Ghousia Akbari MLV Prabhuji Sandip G Chorghade
• 来源期刊: 世界口腔医学杂志
• 学科: 医学
• 关键词: Chair-side test Chronic PERIODONTITIS GINGIVAL crevicular fluid Matrix metalloproteinase-8 PERIODONTAL health
• 年，卷（期）: 2013,（1）
• 页码范围: 24-29
• 页数: 6页
• 分类号: R

摘要

AIM:To validate accuracy of a novel chair-side test for matrix metalloproteinase(MMP)-8 as compared to enzyme-linked immunosorbent assay(ELISA)in Periodontal health and disease.METHODS:Gingival crevicular fluid was collected from 150 subjects,Group 1(healthy)-50 subjects,Group 2(gingivitis)-50 subjects and Group 3(chronic periodontitis)-50 subjects.A chair-side test strip was indigenously prepared using polyclonal antibodies(principle of immunochromatography)to detect the MMP-8 levels.The detection accuracy(sensitivity and specificity)of the MMP-8 levels by chair-side test kit were compared with ELISA at baseline and 3 mo after scaling and root planing among the study population.RESULTS:The novel chair side test detected MMP-8 levels in accordance with ELISA which at baseline were higher in Group 2 and Group 3 as compared to controls(P<0.05),and these enzyme levels decreased after therapy(P<0.05).The chair-side test could differentiate healthy,gingivitis and periodontitis.The detection accuracy of the chair-side test strip were on par with ELISA(sensitivity 92.9%and specificity of 100%)which were statistically significant(P<0.05).A desire to arouse interest about periodontal health and maintenance in the Indian population provided a strong rationale for us to develop our chair-side test strips to suit our economy.Moreover,this was the first ever effort to develop and validate a chair-side test strip to detect MMP-8 levels in the Indian population.This test can be used on a large scale in private dental practice for the early detection of disease,tapping the sites at risk for disease,alongside helps in patient education and motivation for maintenance.CONCLUSION:This study shows that the novel chair side test kit detects MMP-8 levels a biomarker of periodontal disease progression accurately making it a good chair side diagnostic tool.Further,it is cost effective and time saving which can make it applicable in private dental practice on a large scale for the early detection of periodontal disease.