摘要:
分别利用浓度为10、50、100、150 μmol/L的CdCl2处理萝卜幼苗,分析根系全蛋白质组和根系线粒体蛋白质组发生的变化,并对其中100 μmol/L的CdCl2诱导下萝卜根系的差异表达蛋白质组作详细分析,利用质谱技术鉴别差异蛋白的归属.根系全蛋白中有22个蛋白质斑点显示出差异表达,其中11个斑点(斑点4、斑点9~18)下调,4个斑点(斑点19~22)上调,7个斑点(斑点1~3,5~8)被抑制表达.线粒体中有22个蛋白质斑点显示差异表达,其中19个斑点(斑点1~19)下调,2个斑点(斑点20~21)上调,斑点22被诱导合成,经CdCl2诱导后,萝卜根系内的蛋白质组产生明显的差异表达现象,这些蛋白质分别是:cruciferin,maturase K,DNA-binding protein,defence-related protein,F-box family protein,F-actin capping protein beta subunit,transcription factor,monodehydroascorbate reductase,chaperone binding protein,SHLI,RNA polymerase beta chain,ATF3,putative acyl transferase 4,transposon protein putative mutator sub-class,transposon protein putative En/spm sub-class,retransposon protein,putative Tyl-copia sub-class,isocitrate lyase,phytochrome kinase substrate-related,phosphoenolpyruvate carboxylase,serine/threonine-protein kinase,ATP synthase beta chain,ARol-like protein 4,catalase isozyme 1,pentatricopeptide (PPR)repeat-countaining protein,cytochrome p450,DNA polymerase Ⅰ,trihelix transcription factor,NADH dehydrogenase submit F,cycline-dependent kinase,F-box protein-related,ATP synthase subunit beta,mitochondrial precursor,glucan phosphorylase putative,zinc finger family protein,heat shock protein 26,peroxiredoxin type2 putative,alcohol dehydrogenase.这些差异表达的蛋白质,其功能涉及DNA、RNA及蛋白质合成,基因转录、转座与内含子剪接,细胞信号转导与结构维持,以及不良环境条件下的防御与能量代谢等方面.