Optical Rotatory Dispersion Measurement of D-Glucose with Fixed Polarizer Analyzer Accessory in Conventional Spectrophotometer

• 作者: Alfons Penzkofer
• 来源期刊: 分析科学方法和仪器期刊(英文)
• 学科: 物理学
• 关键词: OPTICAL Activity OPTICAL Rotatory Dispersion Specific OPTICAL ROTATION Molar OPTICAL ROTATION POLARIMETER Spectropolarimeter Grape Sugar D-GLUCOSE
• 年，卷（期）: fxkxffhyqqkyw_2013,（4）
• 页码范围: 234-239
• 页数: 6页
• 分类号: O43

摘要

In the sample compartment of a conventional spectrophotometer, mounting of a polarizer before sample and an analyzer behind sample allows the determination of the optical rotatory dispersion of optical active media by measurement of the transmission ratio of crossed and parallel arranged polarizer and analyzer. A formula for the determination of the angle of rotation is derived from the transmission ratio. The arrangement is applied to determine the molar optical rotation of D-glucose in water in the wavelength range from 220 nm to 820 nm.