Research of Quality Standards for Stachydrine Hydrochloride in Chinese Medicine TJF Granule

• 作者: C. Wiart Pingniang Shen T. J. Khoo Wei Yu Yao Lu
• 来源期刊: 药理与制药(英文)
• 学科: 医学
• 关键词: TJF GRANULE Quality Standards Traditional Chinese Medicine EVAPORATIVE Light Scattering Detector (ELSD) High-Performance Liquid CHROMATOGRAPHIC (HPLC) Method Stachydrine HYDROCHLORIDE PHARMACEUTICAL Analysis Determination
• 年，卷（期）: 2013,（3）
• 页码范围: 277-282
• 页数: 6页
• 分类号: R2

摘要

A high-performance liquid chromatographic method was applied to the determination of stachydrine hydrochloride concentration in TJF granule (Chinese name: Tiao-Jing-Fang), using a mobile phase of methanol-acetonitrile (50:50, v/v) by the Agilent Kromasi NH2 column (250 mm × 4.6 mm, 5 μm, S/N: 22N25110). Detection wavelength was 201 nm. The result revealed good linearity of stachydrine hydrochloride and was obtained within the range of 0.20 - 1.98 μg/mL (R = 0.9995). The average recovery was 97.01%;the relative standard deviation (RSD) was 0.19%. To the best of our knowledge, this is the first report dedicated to the determination of stachydrine hydrochloride by the evaporative light scattering detector-high-performance liquid chromatographic (ELSD-HPLC) method.