Comparison of Extraction Methods for Genomic of Fall Webworm [ Hyphantria cunea （Drury）] Amplification Effect Sequences of CO I and of Different Primers CO II and Cytb DNA and on

• 作者: Cai Ping Chen Jingyun Zhan Guohui Zhuang Mingjuan
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 生物学
• 关键词: 基因组DNA 美国白蛾 提取方法 CYTB CO 引物 序列 一氧化碳
• 年，卷（期）: pdapzwbchyj_2013,（4）
• 页码范围: 36-38
• 页数: 3页
• 分类号: Q781

摘要

[Objective] The paper was to compare different extraction methods for genomic DNA of fall webworm [Hyphantria cunea( Drury) ]and study the amplification effect of different primers on sequences of CO I and CO II and Cytb.[Method] With winter pupae of fall webworm as the test materials,genomic DNA of fall webworm were extracted by five different methods including CTAB method,SDS method,improved SDS method,kit method and different processing time.Six groups of different primers were used to amplify sequences of CO I and CO II and Cytb.[Result] Full DNA could be extracted by CTAB method,SDS method and improved SDS method,and the effort of low concentration SDS method was the best.Six groups of primers could amplify the corresponding fragment,and two groups of primers had the best effort for amplification of CO I.[Conclusion] The study provides better methods for DNA extraction.