Fast Counting Research of Escherichia coil in Water Based on Salicylic Acid Culture Solution by High Performance Liquid Chromatography

• 作者: Baoying Lv Wei Liang Yanxian Nong Zhaoyu Mo
• 来源期刊: 气象与环境研究：英文版
• 学科: 化学
• 关键词: 高效液相色谱法 大肠杆菌 快速计数 水杨酸 培养液 中水 二羟基苯甲酸 测定
• 年，卷（期）: 2013,（9）
• 页码范围: 34-35
• 页数: 2页
• 分类号: O657.72

摘要

[Objective]Based on salicylic acid culture solution,high performance liquid chromatography(HPLC)was used to rapidly count E.coli in water.[Method]Because E.coli metabolism could produce polyatomic phenol(mainly 2,5-dihydroxybenzoic acid)in salicylic acid culture solution,and generated amount of polyatomic phenol was proportionate to the number of E.coli,we detected 2,5-dihydroxybenzoic acid by HPLC.Through linear relationship,the number of E.coli was calculated,realizing fast counting of E.coli,and detection time only needed 3 h.[Result]Linearity range of the method detecting E.coli was 5×104-5×1010cells/ml,and the detection limit was 1×104cells/ml.[Conclusion]HPLC method realized rapid counting of E.coli.