在人的透镜在上皮的间充质的转变和细胞外的矩阵合成的正式就职上转变生长因素 2 和结缔组织生长因素的效果上皮的细胞

• 作者: Bo Ma Cheng Pei Li Qin Li-Jun Cui Qian-Yan Kang
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: transforming GROWTH FACTOR β2 CONNECTIVE tissue GROWTH FACTOR posterior CAPSULAR OPACIFICATION human lens epithelial cells extracellular matrix α-smooth muscle actin type Ⅰ collagen fibronectin
• 年，卷（期）: 2013,（6）
• 页码范围: 752-757
• 页数: 6页
• 分类号: R776

摘要

AIM:To Investigate the effects of transforming growth factorβ2(TGF-β2)and connective tissue growth factor(CTGF)on transdifferentiation of human lens epithelial cells(HLECs)cultured in vitro and synthesis of extracellular matrix(ECM).METHODS:HLECs were treated with TGF-β2(0,0.5,1.0,5,10μg/L)and CTGF(0,15,30,60,100μg/L)for different times(0,24,48,72h)in vitro and the expression ofα-smooth muscle actin(α-SMA),the main component of the extracellular matrix typeⅠcollagen(Col-1)and fibronectin(Fn)were measured by using real-time polymerase chain reaction(PCR)and western-blot.RESULTS:TGF-β2 and CTGF significantly increased expression ofα-SMA mRNA and protein(P【0.05,P【0.001),Fn mRNA and protein(P【0.001),Col-1 mRNA and protein(P【0.001).TGF-β2 could induce HLECs expression of CTGF mRNA and protein in dosedependent manner(P【0.05,P【0.001).TGF-β2 and CTGF could induce HLECs to expressα-SMA,Fn and Col-1 in time-dependent manner.Each time of TGF-β2and CTGF induced HELCs expression ofα-SMA,Fn,Col-1 mRNA and protein was significant increase compared with control(P【0.05,P【0.001).CONCLUSION:TGF-β2 and CTGF could induce HLECs epithelial mesenchymal transition and ECM synthesis.