Acetylcholinesterase function in apoptotic retina pigment epithelial cells induced by H_2O_2

• 作者: Hong-Fei Liao Jing-Lin Yi Li Cai Man Xu Xue-Jun Zhang Yi Shao
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: ACETYLCHOLINESTERASE RETINA pigment epithelial cells oxidative stress AGE-RELATED MACULAR DEGENERATION
• 年，卷（期）: 2013,（6）
• 页码范围: 772-777
• 页数: 6页
• 分类号: R774

摘要

AIM:To investigate the acetylcholinesterase（AChE）expression involved in retina pigment epithelial（RPE）apoptosis induced by higher concentrations H₂O₂.METHODS:The human retinal pigment epithelium cell line ARPE-19 was from ATCC（Rockville,MD）.Cultured ARPE-19 cells were treated with H₂O₂ at 0,250,500,1000,2 000μmol/L and cell viability was measured with MTT assay.AChE expression and DNA fragments were analyzed by immunocytochemistry,TUNEL and PARP-1Western blotting.RESULTS:Immunofluorescence detected AChE exist in the normal human retinal tissue.When H₂O₂】500μmol/L,AChE expression showed an increase after 2h,and this concentration was selected for the present study.RPE cell was induced with 1 000μmoI/L H₂O₂ for 2h,compared to the control group,cell activity decline detected by MTT,AChE and PARP-1 protein expression was significantly increased detected by Western blotting.AChE immunofluorescence staining was positive in RPE cell after H_O₂ incubate 2h.In addition,pretreatment with100|jmol/L epigallocatechin gallate（EGCG）,cell viability increased from 31.20%±3.90%to 70.23%±12.96%.CONCLUSION:AChE is weakly expressed in normal human RPE cells.Stimulation with H₂O₂ caused the stable increase of AChE expression in RPE cells,which may indicate that AChE may be an important role in AMD.