A method to synthesize cDNA constructs by homology based recombination cloning

• 作者: Neetu Verma Pradeep Kumar Burma
• 来源期刊: 美国分子生物学期刊(英文)
• 学科: 医学
• 关键词: HOMOLOGY BASED Recombination CLONING CDNA Assembly
• 年，卷（期）: 2014,（1）
• 页码范围: 16-19
• 页数: 4页
• 分类号: R73

摘要

We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.