rDNA Sequencing and Preliminary Analysis of Microorganisms from Diseased Edible Fungus Using Non-culture Technology

• 作者: Gao Neng Gong Zhiyuan Han Jiandong Huang Chun-yan Liu Xiao Ren Haixia Ren Pengfei Wan Luzhang Yao Qiang
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: RDNA序列 食用菌 微生物技术 细菌性疾病 培养 基因组DNA 聚合酶链反应 通用引物
• 年，卷（期）: 2014,（1）
• 页码范围: 13-16
• 页数: 4页
• 分类号: S435.621.2

摘要

The metagenomic DNA of disease tissue samples from four kinds of major edible fungus was extracted by CTAB method combined with DNA gel recovery kit. The genomic DNA was amplified by polymerase chain reaction using the universal primers of 16S rDNA and 18S rDNA,and then monoclonal sequenced after ligated and transformed. rDNA sequences of 20 positive clones were selected randomly from each pair of primers for sequence alignment. The results showed that there were two bacterial diseases and two fungal diseases in the samples,respectively.