An archaeometric analysis of the James Ossuary inscription “James Son of Joseph Brother of Jesus” strengthens the contention that the ossuary and its engravings are authentic. The beige patina can be observed on the surface of the ossuary, continuing gradationally into the engraved inscription. Fine long striations made by the friction of falling roof rocks continuously crosscut the letters. Many dissolution pits are superimposed on several of the letters of the inscription. In addition to calcite and quartz, the patina contains the following minerals: apatite, whewellite and weddelite (calcium oxalate). These minerals result from the biogenic activity of microorganisms that require a long period of time to form a bio-patina. Moreover, the heterogeneous existence of wind-blown microfossils (nannofossils and foraminifers) and quartz within the patina of the ossuary, including the lettering zone, reinforces the authenticity of the inscription.