Evaluation of antibiotic activity produced by Pseudomonas aeruginosa LV strain against Xanthomonas arboricola pv. pruni

• 作者: Admilton Gon?alves de Oliveira amile Priscila de Oliveira Beranger Ane Stefano Simionato Celia Guadalupe Tardeli de Jesus Andrade Fernanda Corrêa da Silva Vasconcellos Flavia Regina Spago Galdino Andrade Jo?o Carlos Palazzo de Mello Juca Abramo Barrera San Martin Juliana Fonteque Pistori Lucilene Lopes-Santos Martha Viviana Torres Cely
• 来源期刊: 农业科学(英文)
• 学科: 医学
• 关键词: PEACH Tree BACTERIAL Spot Biological Control Leaf LESIONS XANTHOMONAS arboricola Pv. pruni
• 年，卷（期）: 2014,（1）
• 页码范围: 71-76
• 页数: 6页
• 分类号: R73

摘要

Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is considered as a major problem in peach orchards. Copper and antibiotics are used to control, and biocontrol should be a new alternative with low environment impact. The objective was evaluated by the antibiotic activity of the metabolite produced by Pseudomonas aeruginosa LV strain. The free cells supernatant was fractionated with a serial organic solvent with crescent polarity and a dichloromethane phase was concentrated and lyophilised, and after was fractionated using vacuum liquid chromatography. The antibiotic activity of the F3 fraction containing an organometallic compound was tested on Xap in vitro and in a greenhouse conditions. Plants were sprayed with F3 before or after Xap infection and the results showed changes in exopolysaccharides and cell morphology. The F3 concentration of 450 μg·mL-1 was more effective. The results showed that F3 fraction could be a new alternative to control bacterial spot.