Sensitivity Evaluation of Two Human Breast Cancer Cell Lines to Tamoxifen through Apoptosis Induction

• 作者: Charles Azuelos Shyamal K. Majumdar Spencer Keene
• 来源期刊: 细胞凋亡(英文)
• 学科: 医学
• 关键词: TAMOXIFEN Apoptosis MCF-7 and MDA-MB-231 Human BREAST Cancer Cell Lines MITOCHONDRIAL Membrane Potential Assay ESTROGEN Receptor
• 年，卷（期）: 2014,（4）
• 页码范围: 70-77
• 页数: 8页
• 分类号: R73

摘要

Tamoxifen citrate (TAM) has been used to treat breast cancer in women for many years. The com-parative effects of TAM in inducing apoptosis were evaluated in estrogen receptor-positive (ER- positive MCF-7) and estrogen receptor-negative (ER-negative MDA-MB-231) human breast cancer cell lines in vitro in order to determine if these two cell lines differ in their sensitivity to TAM. Mi-tochondrial membrane permeability potential disruption was assessed in both cell lines by a lip-ophilic cationic dye (DePsipher assay, Trevigen, Inc.) utilizing fluorescence microscopy. Using this specific fluorochrome, we were able to associate mitochondrial membrane disruption to early, mid-, and late apoptotic cells. TAM induced cell death via apoptosis in both ER-positive and ER- negative cells, however, apoptosis induction was more pronounced in ER-positive MCF-7 compared to ER-negative MDA-MB-231 breast cancer cells. These findings may have some therapeutic use in the treatment of estrogen dependent and estrogen independent breast cancer.