In order to enhance hydrolysis rate of bagasse, ultrasonic-assisted pretreatment is made in the experiment. The influence of addition of sulfuric acid, ultrasonic temperature, power and time to the hydrolysis of bagasse will be discussed in this paper. Optimal process and quadratic regression equation is obtained by using the response surface method. The yield of reducing sugar increases 6.5 percent after ultrasonic-assisted pretreatment. It demonstrates that the ultrasonic-assisted pretreatment can improve the hydrolysis rate of the bagasse.