Antibacterial Activity of Polyethylenimine/Carrageenan Multilayer against Pathogenic Bacteria

• 作者: Annabelle V. Briones Toshinori Sato Ursela G. Bigol
• 来源期刊: 化学工程与科学期刊(英文)
• 学科: 医学
• 关键词: CARRAGEENAN POLYETHYLENIMINE BACTERIAL Growth INHIBITION ANTI-BACTERIAL
• 年，卷（期）: 2014,（2）
• 页码范围: 233-241
• 页数: 9页
• 分类号: R73

摘要

The multilayer of polyethylenimine (PEI) and carrageenan (k, i, l) formed by layer-by-layer assembly was investigated for its antibacterial activity against Enterobacter cloaceae, Staphylococcus aureus and Enterococcus faecalis 29505 for potential use as coating on biomaterial surface. All the multilayers exhibited growth inhibition. PEI/Iota carrageenan multilayer was effective in inhibiting the growth of the E. cloaceae, S. aureus and E. faecalis while PEI/Lambda carrageenan was effective in inhibiting the growth of E. cloaceae. Results of the paper strip test for combined action of carrageenan and PEI showed synergism with regards to bacterial growth inhibition. The multilayers had also contact-killing effect with the test organisms. The multilayer was also characterized by atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and biomolecular interaction analysis.