Cloning of Rice Blast Resistance Gene Pik2 -H4 and Screening of Its Interaction Protein

• 作者: Chen Zhiqiang Liu Hao Sun Dayuan Wang Hui
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 生物学
• 关键词: 稻瘟病抗性基因 相互作用 蛋白质 克隆 酵母双杂交系统 筛选 抗稻瘟病基因 全长序列
• 年，卷（期）: 2014,（4）
• 页码范围: 26-30
• 页数: 5页
• 分类号: Q51

摘要

The broad-spectrum rice blast resistance gene Pik2-H4 was cloned from the highly resistant line H4. The full-length sequence of Pik2-H4 was 3 066 bp,which was different from the corresponding allele sequence of Nipponbare. Pik2-H4 belonged to NBS-LRR genes and coded a protein containing NB-ARC domain and leucine-rich repeats. To find the interaction proteins with Pik2-H4 from the rice,the yeast two-hybrid system was used and three important proteins（ LOCOs08g39300,LOCOs03g25960 and LOC-Os09g29130）were identified. The results could provide some new information for the mechanism of rice blast resistance mediated by Pik2-H4.