Keratinocyte 生长 factor-2 和自体同源的浆液加强处于在老鼠的角膜损坏的间充质的干细胞的再生效果

• 作者: Ferda Alpaslan Pinarli Feride Duru Gülsen kten Leman Tomak Mehmet Kefeli Nurten Kara Tun Fisgin mit Beden
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: CORNEAL wound HEALING MESENCHYMAL stem cells KERATINOCYTE growth factor-2 AUTOLOGOUS serum amniotic membrane
• 年，卷（期）: 2014,（2）
• 页码范围: 211-219
• 页数: 9页
• 分类号: R772.2

摘要

AIM:To investigate the healing process after severe corneal epithelial damage in rats treated with mesenchymal stem cells(MSCs)cultured with or without keratinocyte growth factor(KGF-2)and autologous serum(AS)on amniotic membrane(AM).Many patients are blind and devastated by severe ocular surface diseases due to limbal stem cell deficiency.Bone marrow-derived MSCs are potential sources for cellbased tissue engineering to repair or replace the corneal tissue,having the potential to differentiate to epithelial cells.METHODS:The study included 5 groups each including 10 female'Sprague Dawley'rats in addition to20 male rats used as bone marrow donors.Group I rats received AM+MSCs,Group II rats AM+MSCs cultured with KGF-2,Group III rats AM+MSCs cultured with KGF-2+AS,Group IV rats only AM and Group V rats,none.AS was derived from blood drawn from male rats and bone marrow was obtained from the femur and tibia bones of the same animals.Therapeutic effect was evaluated with clinical,histopathological and immunohistochemical assessment.MSC engraftment was demonstrated via detection of donor genotype(Y+)in the recipient tissue(X)with polymerase chain reaction.RESULTS:Corneal healing was significantly better in Groups I-III rats treated with MSC transplantation compared to Group IV and Group V rats with supportive treatment only.The best results were obtained in Group III rats with 90%transparency,70%lack of neovascularization,and 100%epithelium damage limited to less than 1/4 of cornea.CONCLUSION:We suggest that culture of MSCs with KGF-2 and AS on AM is effective in corneal repair in case of irreversible damage to limbal stem cells.