Development of a Rapid and Efficient Liquid Chromatography Method for Determination of Gibberellin A4 in Plant Tissue, with Solid Phase Extraction for Purification and Quantification

• 作者: Adalberto Benavides-Mendoza Julia Medrano Macías Manuel Humberto Reyes-Valdés Rahim Foroughbakhch Pournavab
• 来源期刊: 美国植物学期刊(英文)
• 学科: 化学
• 关键词: Dasyrilon cedrosanum Epithelantha micromeris PHYTOHORMONE Separation Plant Growth REGULATORS REVERSE PHASE Liquid Chromatography Solid PHASE Extraction
• 年，卷（期）: mgzwxqkyw_2014,（5）
• 页码范围: 573-583
• 页数: 11页
• 分类号: O6

摘要

A new, rapid and efficient reverse phase Liquid Chromatography (RP-LC) method was developed for determination of Gibberellin A4 (GA4) in samples of flower stalk of Dasylirion cedrosanum and vegetative tissue of Epithelantha micromeris. Purification of GA4 was carried out by solid phase extraction (SPE), in Epithelantha micromeris. In the chromatography method was obtaining a retention time of 2.1 min, using Hypersil GOLD C-18 column (100 × 4.6 mm dim and size particle 5 μ), mobile phase 50/50 acetonitrile/water and a flow 1.0 ml/min. Detection was carried out by a UV detector set at 205 nm, and a quantization limit of 0.4 mg/L. The obtained correlation coefficient was 0.995.