Improved Recovery of Erythropoietin and Darbepoetin from Equine Plasma by the Application of a Wheat Germ Agglutinin Mediated Pre-Extraction Prior to Immunoaffinity Chromatography

• 作者: Danny Chua Shawn M. R. Stanley
• 来源期刊: 生命科学与技术进展(英文)
• 学科: 医学
• 关键词: ERYTHROPOIETIN DARBEPOETIN Alfa IMMUNOAFFINITY EXTRACTION Lectin Affinity EXTRACTION Wheat Germ AGGLUTININ Horseracing
• 年，卷（期）: 2014,（7）
• 页码范围: 651-660
• 页数: 10页
• 分类号: R73

摘要

We describe a two-step method that uses wheat germ agglutinin immobilized on Sepharose gel followed by immunoaffinity chromatography (IAC) to extract recombinant human erythropoietin and Darbepoetin from equine plasma. Lectin affinity chromatography was shown to be an effective approach for isolating these epoetins from plasma and in combination with IAC;this method gave superior recovery when compared to the use of the latter technique alone. Moreover, due to the ease with which it can be scaled up, it is particularly well suited for pre-concentrating larger volumes of samples prior to IAC and this provides a facile way of improving the overall sensitivity with which these foreign proteins can be detected in equine plasma.