Diuron is a herbicide widely used in the Middle East;its field application creates many environmental problems. This study investigated the bioremediation of Diuron in soil environment by Cyanobacterial mats collected from Gaza Wadi. Various concentrations of Diuron were injected in water saturated soil samples pre-treated with Cyanobacterial mat for several periods. Percent growth of Jews mallow as a test plant was taken as indicator of biodegradation of Diuron. Results showed that Diuron was degraded in soil and degradation was more pronounced when Diuron was incubated with Cyanobacterial in the irrigation water. Larger applied rate of Cyanobacterial mat did not affect the biodegradation of Diuron. These encouraging results suggest that application of Cyanobacterial mat could be a suitable method to remediate soil pollution.